# Posts

### Don't Deconvolve Your PSF

Seriously, don’t do it, it’s not helpful

### Nyquist Sampling SMLM

Getting really good real resolution in SMLM is really hard.

### Oh no, I squished my OTF!

How to make your images look bad with high NA objectives

### Angle to Position and Back

The back pupil plane of a microscope objective is Fourier conjugate to it’s image plane. We use this fact to perform laser …

### Things I've Forgotten

My basic modus operandi has been to learn and then, if not immediately, soon after forget them. This is a list I maintain of things …

### Conversion Factors

Some useful conversion factors from my graduate school days.

### Derivation of the Transform Limit

Here I derive the somewhat well known formula that for a transform limited pulse the bandwidth duration product is 15 ps cm$^{-1}$