Curiosity,
made useful.

Freeze-frame of whole cells

Correlative three-dimensional super-resolution and block-face electron microscopy of whole vitreously frozen cells

Cells need to compartmentalize thousands of distinct proteins, but the nanoscale spatial relationship of many proteins to overall intracellular ultrastructure remains poorly understood. Correlated light and electron microscopy approaches can help. Hoffman et al. combined cryogenic super-resolution fluorescence microscopy and focused ion beam–milling scanning electron microscopy to visualize protein-ultrastructure relationships in three dimensions across whole cells. The fusion of the two imaging modalities enabled identification and three-dimensional segmentation of morphologically complex structures within the crowded intracellular environment. The researchers observed unexpected relationships within a variety of cell types, including a web-like protein adhesion network between juxtaposed cerebellar granule neurons.

Good vibrations?

What role do vibrational coherences, if any, play in condensed phase photoreactivity? While we have observed vibrational coherences in almost any system where we look hard enough, it hasn’t been clear if these are a cause or consequence of reactivity. In this survey of our own work we argue that for fast reactions (< 10 ps) coordinate, coherent nuclear motion is required.